Journal: Dentistry Journal

Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

doi: 10.3390/dj13110494

Figure Lengend Snippet: Immunohistochemical staining of Spon1 of the apical area of the mesial root after root canal treatment. The control group was filled with PGA only, and the experimental group was filled with PGA and 50 µg/mL BMP-7. ( A , B ) Control group at 14 days postoperatively. ( C , D ) BMP-7 group at 14 days postoperatively. ( E , F ) Control group at 28 days postoperatively. ( G , H ) BMP-7 group at 28 days postoperatively. ( B , D , F , H ) Expanded images of the boxed areas indicated in ( A , C , E , G ), respectively. ( n = 5) ( A ) A few Spon1-positive areas were observed. ( B ) Spon1-positive areas were observed compared to the control group. ( C ) Spon1-positive areas were observed compared to Day 14. ( D ) The most Spon1-positive areas were observed compared to other groups. Scale bars = 100 μm; magnified image Bars = 50 μm. Ce: cementum, AB: alveolar bone. ( I ) The ratio of Spon1-positive area in the apical periodontal ligament calculated as: (Spon1-positive area/Area of interest) × 100%. Asterisks indicate significant differences among groups on the same postoperative day (**: p < 0.01), whereas daggers indicate significant differences between Day 14 and Day 28 within the same group (†: p < 0.05, ††: p < 0.01).

Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

Techniques: Immunohistochemical staining, Staining, Control

Journal: Dentistry Journal

Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

doi: 10.3390/dj13110494

Figure Lengend Snippet: mRNA expression levels at each BMP-7 concentration assessed by qRT-PCR analysis. The expression levels of each mRNA were higher in the 100 ng/mL BMP-7-treated group compared to the control group. ( A ) SPON1 mRNA expression levels. ( B ) CEMP1 mRNA expression levels. ( C ) RUNX2 mRNA expression levels. ( D ) BGLAP mRNA expression levels. ( n = 4) Asterisks indicate significant differences among BMP-7 concentrations on the same day (*: p < 0.05, **: p < 0.01), whereas daggers indicate significant differences between Day 7 and Day 14 within the same BMP-7 concentration (†: p < 0.05, ††: p < 0.01).

Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

Techniques: Expressing, Concentration Assay, Quantitative RT-PCR, Control

Journal: Dentistry Journal

Article Title: Bone Morphogenetic Protein 7 Promotes the Differentiation of Periodontal Ligament Fibroblasts into F-Spondin-Expressing Cementoblast-like Cells During Root Canal Treatment—An In Vivo Rat Pulpectomy Model and In Vitro Human Fibroblast Study

doi: 10.3390/dj13110494

Figure Lengend Snippet: Detection of proteins in HPLFs following treatment at each BMP-7 concentration by Western blot analysis. SPON1 and CEMP1 proteins were qualitatively detected, showing the strongest bands in the 100 ng/mL BMP-7-treated group, followed by the 200 ng/mL BMP-7-treated group, whereas minimal or no expression was observed in the control. Lane 1, 0 ng/mL BMP-7-treated group as a control; lane 2, 100 ng/mL BMP-7-treated group; lane 3, 200 ng/mL BMP-7-treated group. Representative qualitative results are shown.

Article Snippet: Each membrane was blocked for 1 h using Blocking One (Nacalai Tesque, Kyoto, Japan) and was then incubated overnight at 4 °C with the following primary antibodies: SPON1 (1:1000, #40372, Signalway Antibody LLC, College Park, MD, USA), CEMP1 (1:500, ab169514, Abcam) and GAPDH (1:10,000, ab8245, Abcam).

Techniques: Concentration Assay, Western Blot, Expressing, Control

Journal: Biochemical and biophysical research communications

Article Title: Effect of F-spondin on cementoblastic differentiation of human periodontal ligament cells.

doi: 10.1016/j.bbrc.2006.08.142

Figure Lengend Snippet: Fig. 2. F-spondin expression in human periodontal tissues. (A) Checking the accuracy of the anti-SPON1 antibody by Western blot. F-spondin antibody specifically recognizes F-spondin in FLAG-F-spondin transfected 293T cells. (B) Immunohistochemical staining with the anti-SPON1 antibody in human periodontal tissue. High expression of F-spondin was observed in cell located along the root surface (triangles). C, cementum; D, dentin; PL, periodontal ligament; AB, alveolar bone. Original magnification ·40 (a), ·200 (b).

Article Snippet: To examine expression of F-spondin in seven human periodontal tissues cases, the 4.5 lm sections were stained immunohistochemically with an anti-SPON1 antibody (Prosci).

Techniques: Expressing, Western Blot, Transfection, Immunohistochemical staining, Staining